Electrophysiological characteristics of cultured hippocampal cholecystokinin positive neurons

doi:10.3969/j.issn.0529-1356.2013.05.004

Abstract

Abstract:

Objective To explore the electrophysiological properties of cultured hippocampal cholecystokinin (CCK) positive (CCK⁺ ) neurons. Methods The cultured hippocampal CCK⁺ neurons were labeled by an adenovirus encoding CCK promoter and green fluorescent protein (GFP) and then whole cell patch clamp technique was carried on to explore the electrophysiology characteristic of hippocampal CCK⁺ neurons. Results The long diameter of CCK⁺ neurons’cell body was (22.85±0.77)μm, and the short diameter was (16.21±0.42)μm. The membrane electrophysiology parameters of CCK⁺ neurons were as following.
Rest membrane potential was(-55.90±1.30)mV. Membrane capacity was (45.77±2.06)pF. Membrane resistance was (711.00±46.69)MΩ. The action potential firing frequency of cultured hippocampal CCK⁺neurons was (26.17±3.41)Hz. The spontaneous inhibitory postsynaptic currents frequency of CCK⁺ neurons was (5.26±0.71)Hz, which was significant higher compared to the frequency of spontaneous excitatory postsynaptic currents ［(3.24±0.62)Hz］. Conclusion The electrophysiology characteristics of hippocampal CCK⁺ neuron may be colsely related to its modulatory effect in central nervous system.

Key words: Cholecystokinin, Interneuron, Hippocampus, Whole cell patch clamp, Rat

ZHANG Yang-hui WEI Xiao-fei* SI Jun-qiang MA Ke-tao ZHANG Lu-lu WANG Qin-wen . Electrophysiological characteristics of cultured hippocampal cholecystokinin positive neurons[J]. AAS, 2013, 44(5 ): 607-611.

References

［1］ Qin LZh, Zhang FX, Li JL, et al. The relationship between 5-htergic terminals and the vestibulo—parabrachial nucleus projection neurons expressing 5-HT _1Areceptor in the vestibular nuclear complex ［J］. Acta Anatomica Sinica, 2007, 38(4):390-393.(in Chinese)
秦灵芝, 张富兴, 李金莲,等.大鼠前庭神经核复合体内5HT能终末与表达5-HT_1A受体的前庭臂旁核投射神经元之间的联系［J］. 解剖学报, 2007, 38(4): 390-393. 
［2］Freund TF, Buzsaki G. Interneurons of the hippocampus ［J］. Hippocampus, 1996, 6(4): 347-470.
［3］Lacaille JC, Mueller AL, Kunkel DD, et al. Local circuit interactions between oriens/alveus interneurons and CA1 pyramidal cells in hippocampal slices: electrophysiology and morphology ［J］. J Neurosci, 1987, 7(7): 1979-1993.
［4］McBain CJ, Fisahn A, Interneurons unbound ［J］. Nat Rev Neurosci, 2001, 2(1): 11-23.
［5］Chhatwal JP, Hammack SE, Jasnow AM, et al. Identification of cell-type-specific promoters within the brain using lentiviral vectors ［J］. Gene Ther, 2007, 14(7): 575-583.
［6］Jasnow AM, Ressler KJ, Hammack SE, et al. Distinct subtypes of cholecystokinin (CCK)-containing interneurons of the basolateral amygdala identified using a CCK promoter-specific lentivirus ［J］. J Neurophysiol, 2009, 101(3): 1494-1506.
［7］Sotnikov OS, Paramonova NM. Cytoplasmic syncytial connection-one of three forms of interneuronal connection ［J］. Usp Fiziol Nauk, 2010, 41(1): 45-57.
［8］Mascagni F , McDonald AJ. Immunohistochemical characterization of cholecystokinin containing neurons in the rat basolateral amygdale ［J］. Brain Res, 2003, 976(2):171-184.
［9］Portera-Cailliau C, Pan DT, Yuste R. Activity-regulated dynamic behavior of early dendritic protrusions: evidence for different types of dendritic filopodia［J］. J Neurosci, 2003, 23(18): 7129-7142.
［10］Wang Y, Gupta A, Toledo-Rodriguez M, et al. Anatomical, physiological, molecular and circuit properties of nest basket cells in the developing somatosensory cortex［J］. Cereb Cortex, 2002, 12(4 ): 395-410.
［11］Lee SY, F?ldy C, Szabadics J, et al.Cell-type-specific CCK2 receptor signaling underlies the cholecystokinin-mediated selective excitation of hippocampal parvalbumin-positive fast-spiking basket cells ［J］. J Neurosci, 2011, 31(30): 10993-11002.
［12］Gu N, Vervaeke K, Storm JF. BK potassium channels facilitate high-frequency firing and cause early spike frequency adaptation in rat CA1 hippocampal pyramidal cells［J］. J Physiol, 2007, 580(Pt.3): 859-882.
［13］Erisir A, Lau D, Rudy B, et al. Function of specific K(+) channels in sustained high-frequency firing of fast-spiking neocortical interneurons［J］ , J Neurophysiology, 1999 , 82 (5):2476-2489.
［14］Goldberg JA, Wilson CJ. Control of spontaneous firing patterns by the selective coupling of calcium currents to calcium-activated potassium currents in striatal cholinergic interneurons ［J］.J Neurosci,2005, 25(44):10230-10238.
［15］Lee JM, Bae JS,Jin HK. Intracerebellar transplantation of neural stem cells into mice with neurodegeneration improves neuronal networks with functional synaptic transmission ［J］. J Vet Med Sci, 2010, 72(8): 999-1009.
［16］Zhang B, Chen X, Lin Y, et al. Impairment of synaptic plasticity in hippocampus is exacerbated by methylprednisolone in a rat model of traumatic brain injury ［J］. Brain Res, 2011, 1382: 165-172.
［17］Andre J, Zeau B, Pohl M, et al. Involvement of cholecystokininergic systems in anxiety-induced hyperalgesia in male rats: behavioral and biochemical studies ［J］. J Neurosci, 2005, 25(35): 7896-7904.
［18］Wyeth MS, Zhang N, Mody I, et al. Selective reduction of cholecystokinin-positive basket cell innervation in a model of temporal lobe epilepsy［J］. J Neurosci, 2010, 30(26): 8993-9006. 

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